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8-苯胺-1-萘磺酸號：82-76-8   
英文名稱：ANSA；1,8-ANS ；8-Anili-naphthalenesulfonic acid；N-Phenyl-1-naphthylamine-8-sulfonic Acid；Phenyl Acid；N-Phenyl peri acid   
其他名稱：N-苯基-8-萘胺-1-磺酸；8-苯氨基-1-萘磺酸；8-苯胺基-1-萘磺酸；N-苯基-1-萘胺-8-磺酸；N-苯基周位酸；苯基-1-萘胺-8-磺酸；苯基周位酸   
號：82-76-8   
C16H13NO3S=299.34   
級別：BR   
純度：≥98.0%   
熔點：215～217℃   
Insolubles in diluted ammonia：≤0.3%   
性狀(以下信息僅供參考)：灰色至暗綠色或黑色粉末。其鈉鹽為淺綠色至灰色針狀或片狀結(jié)晶,不溶于水，溶于乙醇   
用途：本品僅供科研，不得用于其它用途。(以下用途僅供參考)1,8-ANS 是一種熒光染料，對蛋白質(zhì)的疏水表面具有高親和力。1,8-ANS經(jīng)歷藍移和熒光強度顯著增加后，結(jié)合到低極性區(qū)域的蛋白質(zhì)表面。這些屬性使1,8 - ANS 非常適合用于測定親和力的疏水性配體及其相應(yīng)的結(jié)合蛋白，如具有疏水性的游離脂肪酸的脂肪酸結(jié)合蛋白(FABPs)   
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Function : Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of plaets.
Isoform e3C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Subunit : Homodimer. Interacts with NOSIP and NOSTRIN.
Subcellular Location : Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
Tissue Specificity : Plaets, placenta, liver and kidney.
Post-translational modifications : Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity.
Similarity : Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Database links : UniProtKB/Swiss-Prot: P29474.3 human
UniProtKB/Swiss-Prot: Q62600.4 rat
UniProtKB/Swiss-Prot: P70313.4 mouse
英文名稱  Anti-Phospho-eNOS (Ser632)
中文名稱  磷酸化一氧化氮合成酶3（內(nèi)皮型）抗體
別    名  eNOS (phospho S632); p-eNOS (phospho S632); eNOS (Phospho-632); cNOS; eNOS; Constitutive NOS; EC NOS; ecNOS; Endothelial nitric oxidase synthase; Endothelial nitric oxide synthase; Endothelial nitric oxide synthase 3; Endothelial NOS; Nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase 3; Nitric oxide synthase 3 endothelial cell; Nitric oxide synthase endothelial; nitric oxide synthase, endothelial; NOS 3; NOS III; NOS type III; NOS3; NOSIII; NOS3_HUMAN.
濃    度  1mg/1ml
規(guī) 格  0.1ml/100μg
8-苯胺-1-萘磺酸號：82-76-8抗體來源  Rabbit 
克隆類型  polyclonal
交叉反應(yīng)  Human, Mouse, Rat
產(chǎn)品類型  一抗  磷酸化抗體  
研究領(lǐng)域  腫瘤 細胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 合成與降解 新陳代謝 
蛋白分子量  predicted molecular weight: 133kDa
性    狀  Lyophilized or Liquid
免 疫 原  KLH conjugated Synthesised phosphopeptide derived from mouse eNOS around the phosphorylation site of Ser632
亞    型  IgG
純化方法  affinity purified by Protein A
儲 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產(chǎn)品應(yīng)用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500
（石蠟切片需做抗原修復(fù)）
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user. 
保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, plaet aggregation, and cardiac myocyte function.