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LDB2蛋白抗體;Anti-LDB2抗體簡單介紹:
產品編號:BYK-18203R
英文名稱:Anti-LDB2
中文名稱:LDB2蛋白抗體
別名:Carboxyl terminal LIM domain binding protein 1; Carboxyl terminal LIM domain protein 1; Carboxyl-terminal LIM domain-binding protein 1; CLIM 1; CLIM-1; CLIM1; LDB 1; LDB 2; LDB-2; LDB1; LDB2; LDB2_HUMAN; LIM binding domain 2; LIM domain binding 2; LIM domain binding factor 2; LIM domain binding factor CLIM 1; LIM domain binding factor CLIM1; LIM domain binding protein 2; LIM domain-binding factor CLIM1; LIM domain-binding protein 2; OTTHUMP00000158632; OTTHUMP00000217823; OTTHUMP00000217824; OTTHUMP00000217825; OTTHUMP00000217827.
背景介紹:Genes encoding LIM domain-binding factors were initially isolated in a screen for proteins that physically interact with the LIM domains of nuclear proteins (summarized by Semina et al., 1998 [PubMed 9799849]). These proteins, such as the one encoded by the LDB2 gene, are capable of binding to a variety of transcription factors and are likely to function at enhancers to bring together diverse transcription factors and form higher order activation complexes or to block formation of such complexes (Jurata and Gill, 1997 [PubMed 9315627]). The family of genes encoding LIM domain-binding factors includes 2 members isolated from the mouse, Clim1 (Bach et al., 1997 [PubMed 9192866]) and Clim2/Lbd1/Nli (Agulnick et al., 1996 [PubMed 8918878]; Jurata et al., 1996 [PubMed 8876198]; Bach et al., 1997 [PubMed 9192866]) and their homologs cloned from the frog, chicken, and fly. The fact that LIM domain-binding factors are likely to be involved in the coordination of the transcriptional activity of many diverse factors might implicate them in human phenotypes characterized by multiple affected sites
產品規格:50ul;100ul;200ul;1ml,*
抗體來源 Rabbit or Mouse
克隆類型 Polyclonal or Monoclonal
交叉反應、產品應用 (具體交叉反應與產品應用請或添加客服直接索取)
性 狀 Lyophilized or Liquid
濃 度 1mg/ml
LDB2蛋白抗體;Anti-LDB2抗體產品信息:
冰凍切片的免疫組化染色步驟
- 速凍組織
- 冰凍切片4~8μm,冰凍切片后如不染色,必須吹干,儲存低溫冰箱內,或進行短暫預固定后儲存于-20℃冰箱
- 室溫放置30分鐘后,入4℃丙酮固定10分鐘,也可根據需要選擇其他的固定方式
- PBS沖洗,5分鐘×3次
- 用3%過氧化氫孵育5~10分鐘,消除內源性過氧化物酶的活性
- PBS沖洗,5分鐘×3次
- 下接石蠟切片免疫組化染色操作步驟(滴加一抗開始)
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